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KMID : 1149220180260030195
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Korean Journal of Oriental Medical Prescription
2018 Volume.26 No. 3 p.195 ~ p.205
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Inhibitory Effects of Gardeniae Fructus on Apoptosis induced by 4-HNE in PC-12 Cell
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Han Yong-Soo
Lee Tae-Hee
Kim Youn-Sub
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Abstract
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Objectives : The purpose of this study was to observe the effects of Gardeniae Fructus on 4-HNE-induced apoptosis in PC-12 cell.
Methods : A MTT assay was conducted to observe the cytotoxicity of Gardeniae Fructus on the PC-12 cell viability and the cytoprotective effects of Gardeniae Fructus on PC-12 cell against oxidative stress caused by 4-HNE. And western blot was conducted to observe the expression of Bax, Bcl-2, Caspase-3, TNF-α proteins which are involved in intrinsic and extrinsic apoptosis pathway.
Results : 25, 50, 100, 200 and 400 ㎍/㎖ of Gardeniae Fructus water extract had no cytotoxicity on PC-12 cell. 200 ㎍/㎖ of Gardeniae Fructus water extract had significant cytoprotective effect on PC-12 cell against oxidative stress caused by 4-HNE. The expression of Bax protein in 50, 100 and 200 ㎍/㎖ of Gardeniae Fructus was significantly decreased in PC-12 cell. The expression of Bcl-2 protein in 200 ㎍/㎖ of Gardeniae Fructus was significantly increased in PC-12 cell. The expression of Caspase-3 protein in 100 and 200 ㎍/㎖ of Gardeniae Fructus was significantly decreased in PC-12 cell. The expression of TNF-α protein in 50 ㎍/㎖ of Gardeniae Fructus was significantly decreased in PC-12 cell.
Conclusions : These results suggest that Gardeniae Fructus water extract is effective to protect PC-12 cell from 4-HNE induced apoptosis.
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KEYWORD
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Gardeniae Fructus, 4-HNE, Bax, Bcl-2, Caspase-3, TNF-α
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